Fluorene-9-bisphenol通過(guò)破壞睪丸功能和局部微環(huán)境損害男性生育能力

2024年10月，山東大學(xué)齊魯醫(yī)院婦產(chǎn)科生殖醫(yī)學(xué)中心；山東大學(xué)臨沂市人民醫(yī)院生殖醫(yī)學(xué)科 (Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, 107 Wenhua West Road, Ji’nan, Shandong 250012, China b Department of Reproductive Medicine, Linyi People’s Hospital, Shandong University, No. 27, East Section of Jiefang Road, Lin’yi, Shandong 276003, China) Lan Chao老師研究團(tuán)隊(duì)在《Ecotoxicology and Environmental Safety》上發(fā)表論文：

“Fluorene-9-bisphenol impaired male fertility through disrupting the testicular function and local microenvironment”

“Fluorene-9-bisphenol通過(guò)破壞睪丸功能和局部微環(huán)境損害男性生育能力”

Abstract：

Past studies have observed that BHPF induces multi-organ toxicity, however, whether it induces damage to male reproductive system and the specific mechanism remains unclear. In the present study, male mice were given 0, 2, 10 or 50?mg/kg/day of BHPF by gavage for 35 days to observe its effect on reproductive organ and sperm quality. The results indicated that BHPF decreased sperm count and sperm motility in a dose-dependent manner. Besides, our results demonstrated that BHPF triggered the proliferation inhibition and cell death of germ cells in vivo and in vitro. Also, BHPF reduced the expression of function markers for germ cells, Sertoli cells, and Leydig cells, indicating its damage to function of testis cells. Simultaneously, testicular microenvironment was found to be altered by BHPF, as presented with declined testosterone level and decreased expression of local microenvironment regulators. Overall, our findings indicated the detrimental effects of BHPF on male reproductive function in mice, suggesting testicular function and local microenvironment disturbance as mechanism underlying testicular damage.

摘要：

既往研究發(fā)現(xiàn)BHPF具有多器官毒性，但其是否對(duì)男性生殖系統(tǒng)造成損害及其具體機(jī)制尚不清楚。本研究分別給雄性小鼠灌胃0、2、10、50?mg/kg/d BHPF，觀察其對(duì)生殖器官和精子質(zhì)量的影響。結(jié)果表明，BHPF降低精子數(shù)量和精子活力呈劑量依賴性。此外，我們的研究結(jié)果表明，BHPF在體內(nèi)和體外均可引發(fā)生殖細(xì)胞的增殖抑制和細(xì)胞死亡。此外，BHPF降低了生殖細(xì)胞、支持細(xì)胞和間質(zhì)細(xì)胞功能標(biāo)志物的表達(dá)，表明其對(duì)睪丸細(xì)胞功能的損害。同時(shí)，發(fā)現(xiàn)BHPF改變了睪丸微環(huán)境，表現(xiàn)為睪丸激素水平下降和局部微環(huán)境調(diào)節(jié)因子表達(dá)減少。總之，我們的研究結(jié)果表明BHPF對(duì)雄性小鼠生殖功能的有害影響，提示睪丸功能和局部微環(huán)境干擾是睪丸損傷的機(jī)制。

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