新甾體皂苷的提取及其細(xì)胞毒性研究

2018年3月，第四軍醫(yī)大學(xué)藥學(xué)院藥物研究所；氟氮化工國(guó)家重點(diǎn)實(shí)驗(yàn)室；西安現(xiàn)代化學(xué)研究所 (Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, Xi’an 710032, China；State Key Laboratory of Fluorine & Nitrogen Chemicals, Xi’an 710065, China；Xi’an Modern Chemistry Research Institute, Xi’an 710065, China) Haifeng Tang老師研究團(tuán)隊(duì)在《Molecules》上發(fā)表論文：

“New Steroidal Saponins from the Rhizomes of Paris vietnamensis and Their Cytotoxicity”

“新甾體皂苷的提取及其細(xì)胞毒性研究”

Abstract：

Four new spirostanol saponins, named pavitnosides A-D (1-4), with six known steroidal saponins 5-10 were isolated from the rhizomes of Paris vietnamensis. Their chemical structures were determined based on extensive spectroscopic studies and chemical methods. The aglycones of pavitnoside B and pavitnoside C were not reported in previous work. The cytotoxicity of all saponins was evaluated against human glioblastoma U87MG and U251 cell lines. The new spirostanol saponin 1 displayed weak anti-proliferative activity against U87MG cell line and the known saponins 8 and 9 exhibited significant cytotoxicity against the two tumor cell lines, with IC50 values of 2.16 to 3.14 μM, but did not affect the growth of primary cultures of human astrocytes.

摘要：

從越南梧桐根莖中分離到4個(gè)新的螺甾醇類皂苷，命名為pavitnosides A-D(1-4)， 6個(gè)已知甾體類皂苷5-10。它們的化學(xué)結(jié)構(gòu)是根據(jù)廣泛的光譜研究和化學(xué)方法確定的。前文未報(bào)道過(guò)其苷元B和C的苷元。評(píng)價(jià)了各皂苷對(duì)人膠質(zhì)母細(xì)胞瘤U87MG和U251細(xì)胞系的細(xì)胞毒性。新合成的螺甾醇皂苷1對(duì)U87MG細(xì)胞系具有較弱的抗增殖活性，而已知的皂苷8和皂苷9對(duì)兩種腫瘤細(xì)胞系具有明顯的細(xì)胞毒性，IC50值為2.16 ~ 3.14 μM，但對(duì)人星形膠質(zhì)細(xì)胞原代培養(yǎng)物的生長(zhǎng)沒(méi)有影響。

該論文中，人膠質(zhì)瘤細(xì)胞系U251和U87MG的體外培養(yǎng)是使用Ausbian特級(jí)胎牛血清完成的。欲了解或購(gòu)買Ausbian特級(jí)胎牛血清可以聯(lián)系北京締一生物400-166-8600.